The first issue is exploring the topic of climate change which is increasing the frequency of high temperature shocks and water shortages, pointing to the need to develop novel tolerant varieties of crops. Silvana Francesca et al. reported in the journal of plant biology with results of the exploration of the genetic variability of two genotypes of tomato (LA3120 & E42) to identify candidate genes that could regulate stress responses in response to single and combined abiotic stresses of high temperature and water shortage.
Plant functional traits, pollen viability and physiological (leaf gas exchange and chlorophyll a fluorescence emission measurements), and biochemical (antioxidant content and antioxidant enzyme activity) measurements were carried out and showed how new tomato genetic resources can be a valuable source of traits for adaptation to combined abiotic stresses and should be used in breeding programs to improve stress tolerance in commercial varieties.
The NanaPhotometer® was used in this work to quantify the accumulation of reactive oxygen species (ROS) and antioxidant compounds by measuring the levels of: hydrogen peroxide (525 nm), malondialdehyde (532 nm, 600 nm), ascorbic acid (525 nm), and glutathione.
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