Implen NanoPhotometer®, the most advanced microvolume UV/Vis spectrophotometer
Implen NanoPotometer® 21 CFR Part 11 Compliance
Precision & Lifetime Accuracy Guaranteed
- Microvolume and cuvette option in one instrument
- Stand-alone operation (built-in computer)
- 21 CFR part 11 Compatibility
- Full scan and analysis of 12 samples in 20 seconds
- Maintenance and recalibration free operation
- Ability to also measure organic solvents in a drop
- Integrated blank and sample purity control (help comply with MIQE guidelines for qPCR experiments)
- Built-in vortex
- Built-in Web Application Server with REST API for full integration in LIMS systems and lab networks
- Mobility (battery option)
Video Transcript
Compare to Alternative Technologies
The table below demonstrates a comparison of the NanoDrop™ One/One C vs. our NanoPhotometer® line of spectrophotometers
Features | NanoDrop™ One / One C | Implen NanoPhotometer® N60/NP80 | NanoPhotometer® Benefits |
---|---|---|---|
Instrument Performance | |||
Minimum Sample Volume | 1 µL 2 µl sample size is recommended for protein measurements | 0.3 – 2 μL | ✓ Save precious sample volume |
Pathlength | 0.03 – 1 mm Auto-ranging | Utilizes two fixed path lengths 0.67 and 0.07 mm Fixed path lengths never change over the lifetime of the instrument | ✓ No after-sales costs for recalibration and the user always knows that the device is working properly over the entire lifetime |
Measurement Time | 8 seconds | 2.5 – 4 seconds (full scan 200 – 900 nm) | ✓ Finish experiments faster |
Enclosed Sample Measurement Surfaces | NO Column is exposed to surroundings | YES Enclosed sample measurement surfaces No evaporation Accurate results independent from environmental conditions Ability to measure proteins with detergent buffers without sample build-up | ✓ Higher reproducibility for all kinds of samples. Reproducible results even in warm/dry labs. Inexperienced users do not need to hurry. |
Photometric Range Cuvette | 0 – 1.5 A (with a 10 mm cuvette) | 0 – 2.6 A | ✓ No need to dilute high-density samples saves time and avoids manual dilution errors |
Analyze Organic Solvent Samples in a Drop | Cuvette only | The NanoPhotometer® Sample Compression Technology allows the analysis of organic solvent samples in a drop and in a cuvette | ✓ NanoPhotometer can be used for all kinds of samples like those diluted in buffers containing detergents |
Detection Range* | dsDNA: 2 – 27,500 ng/µl BSA: 0.06 – 820 mg/ml | dsDNA: 1 – 16,500 ng/µl BSA: 0.03 – 478 mg/ml | ✓ Greater precision for low concentrations |
Optical Specifications | |||
Measurement Wavelength | Full scan from 190 – 850 nm | Full scan from 200 – 900 nm | ✓ Broader scan range enables the use of NIR dyes |
Illuminated Read Head | N/A | Illuminated read head for an easy sample application | ✓ Helps users to properly position the sample and identify air bubbles for better results |
Light Source | Xenon Flash Lamp | Xenon Flash Lamp | N/A |
Instrument Features | |||
Built-In Vortexter | N/A | YES 2,800 rpm; tube size up to 2.0 ml | ✓ Save money and space for a separate vortex. Never forget to vortex your samples which increases reproducibility. |
Reconditioning-free | NO The pedestal of the instrument may become unconditioned over time, especially when measuring proteins or samples that contain surfactants or detergents. This requires reconditioning of the unit’s pedestal. | YES Scratch-resistant inert quartz pedestal that requires no reconditioning | ✓ Save money and don’t worry about the instrument becoming inaccurate over time |
Quality Control Warnings | Acclaro Technology with contaminant calculations | Sample Control™: quality control technology identifies sample impurities, potential contaminants, turbidity and lint residue Blank Control™: prevents inaccurate readings caused by high background blanks or insufficient cleaning | ✓ No manipulation of the data and misinterpretation of the downstream impact of possible contaminants |
Built-in Web Application Server | NO Limited Android-based internal computer only | Full Web Application Server with REST API | ✓ Flexible integration into any LIMS saves time and prevents manual errors during data transfer |
21 CFR part 11 | By external computer | Stand-alone and by external computer | ✓ Complying with FDA 21 CFR part 11 requirements without the need for additional computers saves time and bench space. Transparent long-term costs due to one-time license purchase and free updates. |
Processing Power, Compatibility, Data Processing | |||
Operating System | Android | Linux based NPOS (NanoPhotometer Operating System) Open platform to connect to Windows, Mac OS, Android, iOS via web application interface | ✓ Don’t worry about malware and connect to any external device |
Internal Data Storage | 32 GB | 64 GB | ✓ Store millions of measurements on the device. The autosave function ensures no data is lost. |
Software Compatibility | Compatible with Windows only | Compatible with Windows 7, 8, 10 (32 and 64 bit), Mac OS X, iOS and Android OS | ✓ Connect to any external device |
Data Transfer and Connectivity Options | The unit includes 3x USB A, Ethernet, Bluetooth, WiFi | The unit includes 2x USB A, USB B, HDMI, Ethernet, WiFi | ✓ Show instrument screen to a broader audience via HDMI e.g. in teaching labs |
Data Output | Separate as .csv .tsv .sql | Complete data as PDF & Excel | ✓ Secure PDF files and all data and graphs containing Excel files enable fast and easy data transfer |
Instrument Control | The device can be connected to a computer via Ethernet cable for operation, control and data storage. | Easy control of the NanoPhotometer is possible via Wi-Fi from tablets or smartphones (Android OS & iOS). Control from PCs via LAN, WiFi or USB (Windows & Mac). The HDMI port allows for presentations directly from the device. | ✓ Flexible control of the instrument with any external device |
WiFi Hotspot | Client possible | Built-in WiFi hotspot and with WiFi client capability | ✓ Data transfer via WiFi independent from surrounding networks |
Instrument Dimensions | 20 × 25.4 × 32.3 cm (8 × 10 × 12.7 inches) | 20 x 20 x 12 cm (7.9 x 7.9 x 4.7 inches) | ✓ Smallest footprint saves precious bench space |
The table below demonstrates a comparison of the spectrophotometers 8-channel NanoDrop™ Eight vs. 12-channel NanoPhotometer® N120
Features | NanoDrop™ Eight | Implen NanoPhotometer® N120 | NanoPhotometer® Benefits |
---|---|---|---|
Instrument Performance | |||
Number of Samples | Up to 8 | Up to 12 Single sample mode available | ✓ Use either 8- or 12-channel pipette and process 96-well plates both vertically or horizontally |
Minimum Sample Volume | 1 – 2 µL 2 µl sample size is recommended for protein measurements | 2 – 3.5 μL | ✓ Better drop stability with 2 µl |
Pathlength | 0.1, 0.2 and 1 mm | Utilizes two fixed path lengths 1 and 0.125 mm Fixed path lengths never change over the lifetime of the instrument | ✓ No after-sales costs for recalibration and the user always knows that the device is working properly over the entire lifetime |
Measurement Time | Less than 20 seconds for 8 samples (full scan 190 – 850 nm) | 20 seconds for 12 samples (full scan 200 – 900 nm) | ✓ Finish experiments faster with increased throughput of 50% |
Enclosed Sample Measurement Surfaces | NO Column is exposed to surroundings | YES Enclosed sample measurement surfaces No evaporation Accurate results independent from environmental conditions Ability to measure proteins with detergent buffers without sample build-up | ✓ Higher reproducibility for all kinds of samples. Reproducible results even in warm/dry labs. Inexperienced users do not need to hurry. |
Analyze Organic Solvent Samples in a Drop | N/A | The NanoPhotometer® Sample Compression Technology allows the analysis of organic solvent samples in a drop | ✓ NanoPhotometer can be used for all kinds of samples like those diluted in buffers containing detergents |
Detection Range* | dsDNA: 2 – 10,000 ng/µl BSA: 0.06 – 280 mg/ml | dsDNA: 2 – 8,000 ng/µl BSA: 0.06 – 230 mg/ml | N/A |
Optical Specifications | |||
Measurement Wavelength | Full scan from 190 – 850 nm | Full scan from 200 – 900 nm | ✓ Broader scan range enables the use of NIR dyes |
Illuminated Read Head | N/A | Illuminated read head for an easy sample application | ✓ Helps users to properly position the sample and identify air bubbles for better results |
Light Source | Xenon Flash Lamp | Xenon Flash Lamp | N/A |
Instrument Features | |||
Quality Control Warnings | Acclaro Technology with contaminant calculations | Sample Control™: quality control technology identifies sample impurities, potential contaminants, turbidity and lint residue Blank Control™: prevents inaccurate readings caused by high background blanks or insufficient cleaning | ✓ No manipulation of the data and misinterpretation of the downstream impact of possible contaminants |
Built-in Web Application Server | N/A | Full Web Application Server with REST API | ✓ Flexible integration into any LIMS saves time and prevents manual errors during data transfer |
21 CFR part 11 | External computer | Stand-alone and by external computer | ✓ Complying with FDA 21 CFR part 11 requirements without the need for additional computers saves time and bench space. Transparent long-term costs due to one-time license purchase and free updates. |
Processing Power, Compatibility, Data Processing | |||
Operating System | N/A Needs to be connected to a Windows computer | Linux based NPOS (NanoPhotometer Operating System) Open platform to connect to Windows, Mac OS, Android, iOS via web application interface | ✓ Don’t worry about Windows malware or discontinued support for old versions |
Onboard Processor | N/A | Built-in computer with high-performance Intel Celeron dual core 2.4 GHz processor | N/A |
Internal Data Storage | N/A | 128 GB | ✓ Store millions of measurements on the device. The autosave function ensures no data is lost. |
Software Compatibility | Compatible with Windows only | Compatible with Windows 7, 8, 10 (32 and 64 bit), Mac OS X, iOS and Android OS | ✓ Flexible control of the instrument with any external device |
Data Transfer and Connectivity Options | USB | The unit includes 2x USB A, USB B, HDMI, Ethernet, WiFi | ✓ Integrate the NanoPhotometer® in your network and transfer data directly from the instrument |
Data Output | Separate as .csv .tsv .sql | Complete data as PDF & Excel | ✓ Secure PDF files and all data and graphs containing Excel files enable fast and easy data transfer |
Instrument Control | The device must be connected to a computer via USB cable for operation, control and data storage. | Easy control of the NanoPhotometer is possible via Wi-Fi from tablets or smartphones (Android OS & iOS). Control from PCs via LAN, WiFi or USB (Windows & Mac). The HDMI port allows for presentations directly from the device. | ✓ Touchscreen operation removes the need for a costly computer and saves bench space |
WiFi Hotspot | N/A | Built-in WiFi hotspot and with WiFi client capability | ✓ Data transfer via WiFi independent from surrounding networks |
Instrument Dimensions | 24 × 33 cm (9.4 × 13 inches) | 20 x 20 x 12 cm (7.9 x 7.9 x 4.7 inches) | ✓ Smallest footprint saves precious bench space |
The table below demonstrates a comparison of the spectrophotometers 8-channel NanoDrop™ 8000 vs. 12-channel NanoPhotometer® N120
Features | NanoDrop™ 8000 | Implen NanoPhotometer® N120 | NanoPhotometer® Benefits |
---|---|---|---|
Instrument Performance | |||
Number of Samples | Up to 8 | Up to 12 Single sample mode available | ✓ Use either 8- or 12-channel pipette and process 96-well plates both vertically or horizontally |
Minimum Sample Volume | 1 µL 2 µl sample size is recommended for protein measurements | 2 – 3.5 μL | ✓ Better drop stability with 2 µl |
Pathlength | 0.2 and 1 mm | Utilizes two fixed path lengths 1 and 0.125 mm Fixed path lengths never change over the lifetime of the instrument | ✓ No after-sales costs for recalibration and the user always knows that the device is working properly over the entire lifetime |
Measurement Time | Less than 20 seconds for 8 samples (full scan 220 – 750 nm) | 20 seconds for 12 samples (full scan 200 – 900 nm) | ✓ Finish experiments faster with increased throughput of 50% |
Enclosed Sample Measurement Surfaces | NO Column is exposed to surroundings | YES Enclosed sample measurement surfaces No evaporation Accurate results independent from environmental conditions Ability to measure proteins with detergent buffers without sample build-up | ✓ Higher reproducibility for all kinds of samples. Reproducible results even in warm/dry labs. Inexperienced users do not need to hurry. |
Analyze Organic Solvent Samples in a Drop | N/A | The NanoPhotometer® Sample Compression Technology allows the analysis of organic solvent samples in a drop | ✓ NanoPhotometer® can be used for all kinds of samples like those diluted in buffers containing detergents |
Detection Range* | dsDNA: 2.5 – 3,700 ng/µl BSA: 0.15 – 100 mg/ml | dsDNA: 2 – 8,000 ng/µl BSA: 0.06 – 230 mg/ml | ✓ No need to dilute higher concentrated samples saves time and eliminates manual dilution errors |
Optical Specifications | |||
Measurement Wavelength | Full scan from 220 – 750 nm | Full scan from 200 – 900 nm | ✓ Broader scan range enables the use of peptides and NIR dyes |
Illuminated Read Head | N/A | Illuminated read head for an easy sample application | ✓ Helps users to properly position the sample and identify air bubbles for better results |
Light Source | Xenon Flash Lamp | Xenon Flash Lamp | N/A |
Instrument Features | |||
Quality Control Warnings | N/A | Sample Control™: quality control technology identifies sample impurities, potential contaminants, turbidity and lint residue Blank Control™: prevents inaccurate readings caused by high background blanks or insufficient cleaning | ✓ No manipulation of the data and misinterpretation of the downstream impact of possible contaminants |
Built-in Web Application Server | N/A | Full Web Application Server with REST API | ✓ Flexible integration into any LIMS saves time and prevents manual errors during data transfer |
21 CFR part 11 | N/A | Stand-alone and by external computer | ✓ Complying with FDA 21 CFR part 11 requirements without the need for additional computers saves time and bench space. Transparent long-term costs due to one-time license purchase and free updates. |
Processing Power, Compatibility, Data Processing | |||
Operating System | N/A Needs to be connected to a Windows computer | Linux based NPOS (NanoPhotometer Operating System) Open platform to connect to Windows, Mac OS, Android, iOS via web application interface | ✓ Don’t worry about Windows malware or discontinued support for old versions |
Onboard Processor | N/A | Built-in computer with high-performance Intel Celeron dual core 2.4 GHz processor | N/A |
Internal Data Storage | N/A | 128 GB | ✓ Store millions of measurements on the device. The autosave function ensures no data is lost. |
Software Compatibility | Compatible with Windows only | Compatible with Windows 7, 8, 10 (32 and 64 bit), Mac OS X, iOS and Android OS | ✓ Flexible control of the instrument with any external device |
Data Transfer and Connectivity Options | USB | The unit includes 2x USB A, USB B, HDMI, Ethernet, WiFi | ✓ Integrate the NanoPhotometer® in your network and transfer data directly from the instrument |
Data Output | Separate as .csv .tsv .sql | Complete data as PDF & Excel | ✓ Secure PDF files and all data and graphs containing Excel files enable fast and easy data transfer |
Instrument Control | The device must be connected to a computer via USB cable for operation, control and data storage. | Easy control of the NanoPhotometer is possible via Wi-Fi from tablets or smartphones (Android OS & iOS). Control from PCs via LAN, WiFi or USB (Windows & Mac). The HDMI port allows for presentations directly from the device. | ✓ Touchscreen operation removes the need for a costly computer and saves bench space |
WiFi Hotspot | N/A | Built-in WiFi hotspot and with WiFi client capability | ✓ Data transfer via WiFi independent from surrounding networks |
Instrument Dimensions | 24 × 32 cm (9.4 × 12.6 inches) | 20 x 20 x 12 cm (7.9 x 7.9 x 4.7 inches) | ✓ Smallest footprint saves precious bench space |
* dsDNA tends to precipitate/coagulate at concentrations above 10,000 ng/µl and should be diluted.
NanoDrop™ and NanoDrop™ One are registered trademarks of Thermo Fisher Scientific.
Implen® is not affiliated, sponsored or endorsed by Thermo Fisher Scientific or NanoDrop™.
Implen's Overcoming Limitations of Other Technologies
Have you experienced any of these difficulties with your current technology?
- Inconsistent protein readings (caused by a drop rupture/low surface tension)
- Instability of drops (caused by used up hydrophobic condition of pedestal)
- Costly recalibration/maintenance or decreasing accuracy over time
- Instrument’s service and support discontinued and software not compatible with Windows10 or Mac OS
- Low reproducibility (mainly caused by evaporation effects)
- Device cannot be cleaned with ethanol and/or operated under laminar flow
- Getting results for more than 40 samples per run is time consuming
- No user management/21 CFR part 11 compliant software available
- Waited for weeks to get instrument repaired
How about trying a device that overcomes all of these issues?
Implen NanoPhotometers® Reviews
“Excellent design and is easy to use”
Rating: 5.0 ★★★★★
Application Area: Neurosciences
“The photometer has excellent design, and is easy to use. Built-in vortex is one of the smartest decisions I’ve seen in lab equipment. Data manipulaton and interface decrease hand-on time, and are user-friendly.”
Olga Starkina Organization: Melsytech LLC, Russia
“Really impressed with the accuracy, ease of use, and increased sample throughput”
Rating: 5.0 ★★★★★
Application Area: DNA Analysis
“I’ve really been impressed with the accuracy, ease of use, and increased sample throughput with this instrument. All this in such a small and sleek black box. The touch screen interface is a nice feature and very straightforward. You can easily navigate through the applications, load templates, access and analyze your data. I like that you’re given the option to run up to 12 samples at a time but you can also choose to run 1 or 2 samples, customizable to your experiments. Having options is always a plus and you get that with the N120. The pipette guide makes it really easy to apply your samples. I can see that a lot of time, expertise, and careful considerations went into developing the NanoPhotometer.”
Nam Che Organization: UCLA Department of Medicine, USA
“Accurate Measurements, Solid Device”
Rating: 5.0 ★★★★★
Application Area: DNA Concentration Quantification
“My lab does a lot of molecular cloning work for synthetic biology projects. For the longest time we didn’t have any reliable and simple way of quantifying DNA concentration. This device has been a a life saver. It is accurate (checked samples against other similar devices), easy to use, has lots of extra features that the competitors lack and is cheaper. If you are just needing to quantify DNA concentration the N50 is a solid choice. But it comes with so many other features you’ll be left wondering how other manufacturers get away with charging more for less. Overall the device is very sturdy and produces accurate results. But beyond just the basics you can do protein and kinetics assays and more. The touchscreen is easy an intuitive. Results are saved directly to the machine as a pdf or spreadsheet and can be retrieved remotely by a tablet/phone or a desktop computer. I prefer to simply copy the pdf directly to my laptop over the network. Another great feature is that the sample pedestal has a visible light shining through it while you load the sample which makes it easier to see what you’re doing. That is something I have not seen on any other microvolume spectrophotometer. My only complaint, if I have to come up with one, is that typing on the touchscreen with my arm extended out in front of my is kind of clumsy. But its a minor inconvenience. I just stick to short samples names.”
Cory Tobin Organization: TheLab Inc, USA
“Best small volume spec on the market”
Rating: 5.0 ★★★★★
Application Area: Protein/nucleic acid quantitation
“The Implen NanoPhotometer N50 is extremely easy to use (intuitive menus and settings), accurate, and genuinely capable of measuring very small volumes repeatably. The touch screen works well even with gloves. Window configurations are adaptable so you can customize the information you’re looking at while working. The instrument is light and mobile, and since it’s all-in-one, you can literally move it to where you’re working as needed. It’s not stuck in a particular corner of the lab. In my opinion, it’s the best nano-scale measurement device on the market for routine lab sample quantification and spectral reading.”
David Rawling Organization: Inflammatix, Inc., USA
“Easy use and accurate results “
Rating: 4.7 ★★★★★
“We are using it for nucleic acid quantification. It’s so easy to use, gives accurate results and easy to save data in organized way. Would recommend it.”
Dr. Assala Samad Organization: University of Oxford, United Kingdom
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NanoDrop™ and NanoDrop™ One are registered trademarks of Thermo Fisher Scientific.
Implen® is not affiliated, sponsored or endorsed by Thermo Fisher Scientific or NanoDrop™.